Mass-based biocatalyst metrics to guide protein engineering and bioprocess development

Research , Development , and Engineering Metrics

We seek to understand how the use of Research, Development, and Engineering (R,D&E) metrics can lead to more effective management of R,D&E. This paper combines qualitative and quantitative research to understand and improve the use of R,D&E metrics. Our research begins with interviews of 43 representative Chief Technical Officers, Chief Executive Offices, and researchers at 10 research-intensiv...

Bioprocess development to improve foreign protein production from recombinant Streptomyces.

Bioprocessing strategies to improve production of the heterologous protein parathion hydrolase from recombinant Streptomyces lividans were investigated. Initial limitations to increased production were overcome by using large amounts of nutrients and feeding these nutrients throughout the fermentation. Batch addition of such large amounts of nutrients resulted in byproduct acid accumulation. Ou...

Bioprocess Engineering – Bioprocess Analysis through Calorimetry and Biothermodynamics

Characterization of a novel stable biocatalyst obtained by protein engineering.

Protein engineering is a powerful tool for the improvement of the properties of biocatalysts. Previously we have applied protein engineering technologies to obtain an extremely stable variant of the thermolysin-like protease from Bacillus stearothermophilus [Van den Burg, Vriend, Veltman, Venema and Eijsink (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 2056-2060]. This variant is much more resistant...

Laboratory of BioProcess Systems Engineering

Laboratory of BioProcess Systems Engineering (BPSE) has been organized by Prof. Kino-oka since April 2009. The laboratory mission is to bring a good fortune in human life through the elucidation and utilization of “bio-potential” by understanding sequential biological events (BioProcess) in the reaction field (Systems). The main stream in BPSE, as shown in Fig.1, is the bioprocess designs in st...